Posttreatment Follow-Up of Brucellosis by PCR Assay

Posttreatment follow-Up of brucellosis by PCR assay.

In order to evaluate the usefulness of a peripheral blood PCR assay in the posttreatment follow-up of brucellosis, a cohort of 30 patients was studied by means of blood cultures, rose Bengal, seroagglutination, Coombs' antibrucella tests, and PCR assay at the time of diagnosis, at the end of treatment, and 2, 4, and 6 months later. Of the 29 patients whose PCR assays were initially positive, 28...

PCR assay for diagnosis of human brucellosis.

We have read with interest the recent article by Morata et al. reporting their study of two of the possible factors which can interfere with specific DNA amplification in a peripheralblood PCR assay used for the diagnosis of human brucellosis (2). The study deals with the optimization of a method described in a previous work by these authors (4), in which they report a sensitivity of 100% and a...

Evaluation of an immunoenzymatic assay detecting specific anti-Toxocara immunoglobulin E for diagnosis and posttreatment follow-up of human toxocariasis.

In order to complete the immunodiagnosis of human toxocaral disease, an immunoenzymatic assay with excretory-secretory antigens from Toxocara canis larvae was developed for the detection of specific immunoglobulin E (sIgE enzyme-linked immunosorbent assay [ELISA]). The specificity of the assay was evaluated in patients presenting with various allergic or helminthic diseases. The sensitivity was...

Diagnosis of recent and relapsed cases of human brucellosis by PCR assay

BACKGROUND Brucellosis affects human populations in many developing countries including the Middle East, and Latin America where it is still endemic. It has been prevalent in Jordan for years, where 7842 cases of human brucellosis were registered at the Ministry of Health during 10 year-period. This study was initiated by the recent increase in the number of human cases diagnosed in a rural are...

Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR